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                                 UNITED STATES                                  
                       SECURITIES AND EXCHANGE COMMISSION                       
                             Washington, D.C. 20549                             
                                      FORM                                      
                                      10-Q                                      
(Mark One)


                                                                                         
  QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934


                         For the quarterly period ended                         
                                   March 31,                                    
                                      2023                                      
                                       or                                       


                                                                                          
  TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934


                       For the transition period from to                        
                             Commission File Number                             
                                   001-38787                                    
                          CYCLERION THERAPEUTICS, INC.                          
             (Exact Name of Registrant as Specified in its Charter)             


                                                             
             Massachusetts                    83-1895370     
    (State or other jurisdiction of        (I.R.S. Employer  
     incorporation or organization)       Identification No.)
                                                             
            245 First Street                     02142       
                   ,                          (Zip Code)     
                   18                                        
                   th                                        
                 Floor                                       
                   ,                                         
               Cambridge                                     
                   ,                                         
             Massachusetts                                   
(Address of principal executive offices)                     

                                                                                
                                       (                                        
                                      857                                       
                                       )                                        
                                    327-8778                                    
               Registrants Telephone Number, Including Area Code                
Securities registered pursuant to Section 12(b) of the Act:


                                                                                        
   Title of each class      Trading Symbol(s)  Name of each exchange on which registered
Common Stock, no par value        CYCN                            The                   
                                                                Nasdaq                  
                                                          Capital Market LLC            


Indicate by check mark whether the registrant (1) has filed all reports 
required to be filed by Section 13 or 15(d) of the Securities Exchange Act of 
1934 during the preceding 12 months (or for such shorter period that the 
registrant was required to file such reports), and (2) has been subject to 
such filing requirements for the past 90 days.
Yes
No
Indicate by check mark whether the registrant has submitted electronically 
every Interactive Data File required to be submitted pursuant to Rule 405 of 
Regulation S-T ((s) 232.405 of this chapter) during the preceding 12 months 
(or for such shorter period that the registrant was required to submit such 
files).
Yes
No
Indicate by check mark whether the registrant is a large accelerated filer, an 
accelerated filer, a non-accelerated filer, smaller reporting company, or an 
emerging growth company. See the definitions of large accelerated filer, 
accelerated filer, smaller reporting company, and emerging growth company in 
Rule 12b-2 of the Exchange Act.


                                                  
Large accelerated filer          Accelerated filer
                                                  
  Non-accelerated filer  Smaller reporting company
                                                  
                                                  
                           Emerging growth company
                                                  


If an emerging growth company, indicate by check mark if the registrant has 
elected not to use the extended transition period for complying with any new 
or revised financial accounting standards provided pursuant to Section 13(a) 
of the Exchange Act.

Indicate by check mark whether the registrant is a shell company (as defined 
in Rule 12b-2 of the Exchange Act). Yes

No
As of May 1, 2023
, the registrant had
43,524,894
shares of common stock, no par value, outstanding.

-------------------------------------------------------------------------------

                        CYCLERION PHARMACEUTICALS, INC.                         
                         QUARTERLY REPORT ON FORM 10-Q                          
                      FOR THE QUARTER ENDED MARCH 31, 2022                      
                               TABLE OF CONTENTS                                


                                                                                                                                   
                                                                                                                               Page
                                                                                                 PART I  FINANCIAL INFORMATION     
 Item 1.                                                                                      Financial Statements (unaudited)    5
                                              Condensed Consolidated Balance Sheets as of March 31, 2023 and December 31, 2022    5
         Condensed Consolidated Statements of Operations and Comprehensive Loss for Three Months Ended March 31, 2023 and 2022    6
                       Condensed Consolidated Statements of Stockholders Equity for Three Months Ended March 31, 2023 and 2022    7
                                Condensed Consolidated Statements of Cash Flows for Three Months Ended March 31, 2023 and 2022    9
                                                                      Notes to the Condensed Consolidated Financial Statements   10
 Item 2.                                  Managements Discussion and Analysis of Financial Condition and Results of Operations   22
 Item 3.                                                            Quantitative and Qualitative Disclosures About Market Risk   30
 Item 4.                                                                                               Controls and Procedures   30
                                                                                                                                   
                                                                                                    PART II  OTHER INFORMATION     
 Item 1.                                                                                                     Legal Proceedings   31
Item 1A.                                                                                                          Risk Factors   31
 Item 5.                                                                                                     Other Information   31
 Item 6.                                                                                                              Exhibits   31
                                                                                                                    Signatures   33





-------------------------------------------------------------------------------

              CAUTIONARY NOTE REGARDING FORWARD-LOOKING STATEMENTS              
This Quarterly Report on Form 10-Q contains forward-looking statements within 
the meaning of the federal securities laws, which statements involve 
substantial risks and uncertainties. All statements in this report, other than 
statements of historical facts, including statements about future events, 
financing plans, financial position, business strategy, budgets, projected 
costs, plans and objectives of management for future operations, are 
forward-looking statements that involve certain risks and uncertainties. Use 
of the words may, might, will, would, could, should, believes, estimates, 
projects, potential, expects, plans, seeks, intends, evaluates, pursues, 
anticipates, continues, designs, impacts, affects, forecasts, target, outlook, 
initiative, objective, designed, priorities, goal or the negative of those 
words or other similar expressions may identify forward-looking statements 
that represent our current judgment about possible future events, but the 
absence of these words does not necessarily mean that a statement is not 
forward-looking.
Forward-looking statements are based on our current expectations and 
assumptions regarding our business, the economy and other future conditions. 
Because forward-looking statements relate to the future, by their nature, they 
are subject to inherent uncertainties, risks and changes in circumstances that 
are difficult to predict. As a result, our actual results may differ 
materially from those contemplated by the forward-looking statements. 
Important factors that could cause actual results to differ materially from 
those in the forward-looking statements include regional, national, or global 
political, economic, business, competitive, market and regulatory conditions 
and the following:
"
we could be delisted from Nasdaq;
"
there is substantial doubt regarding our ability to continue as a going concern;
"
the timing, investment and associated activities involved in developing, 
obtaining regulatory approval for, launching and commercializing our product 
candidates, including zagociguat and CY3018;
"
our relationships with third parties, collaborators and our employees;
"
our ability to execute our strategic priorities;
"
our ability to finance our operations and business initiatives;
"
the success of collaboration or license agreements of our product candidates;
"
our ability to access capital, capabilities, and transactions necessary to 
advance the development of our assets;
"
our ability to pursue a transaction or that any transaction, if pursued, will 
be completed on attractive terms, including in particaular whether the Asset 
Purchase Agreement (as defined below in Note 1 to the Condensed Consolidated 
Financial Statements) will be consummated in accordance with its terms;
"
whether the praliciguat out-license will result in the creation of any 
therapies;
"
whether any development, regulatory, and commercialization milestones or 
royalty payments provided for in the agreement with Akebia (as defined below 
in Note 1 to the Condensed Consolidated Financial Statements) will be achieved;

"
the impact on our business of workforce and expense reduction initiatives;
"
our plans with respect to the development, manufacture or sale of our product 
candidates and the associated timing thereof, including the design and results 
of pre-clinical and clinical studies;
"
the safety profile and related adverse events of our product candidates;
"
the efficacy and perceived therapeutic benefits of our product candidates, 
their potential indications and their market potential;
"
U.S. and non-U.S. regulatory requirements for our product candidates, 
including any post-approval development and regulatory requirements, and the 
ability of our product candidates to meet such requirements;
                                                                                
                                       3                                        
-------------------------------------------------------------------------------

"
our ability to attract and retain employees needed to execute our business 
plans and strategies and our ability to manage the impact of any loss of key 
employees;
"
our ability to obtain and maintain intellectual property protection for our 
product candidates and the strength thereof;
"
our future financial performance, revenues, expense levels, payments, cash 
flows, profitability, tax obligations, capital raising and liquidity sources, 
real estate needs and concentration of voting control, as well as the timing 
and drivers thereof, and internal control over financial reporting;
"
our ability to compete with other companies that are or may be developing or 
selling products that are competitive with our product candidates;
"
the impact of government regulation in the life sciences industry, 
particularly with respect to healthcare reform;
"
the coronavirus ("COVID-19") pandemic may continue to disrupt our business, 
including our development activities; and
"
trends and challenges in the markets for our potential products.
See the Risk Factors section in Part I, Item 1A of our Annual Report on Form 
10-K for the fiscal year ended December 31, 2022, and elsewhere in this 
Quarterly Report on Form 10-Q for a further description of these and other 
factors. We caution you that the risks, uncertainties, and other factors 
referenced above may not contain all of the risks, uncertainties and other 
factors that are important to you. In addition, we cannot assure you that we 
will realize the results, benefits, or developments that we expect or 
anticipate or, even if substantially realized, that they will result in the 
consequences or affect us or our business in the way expected. There can be no 
assurance that (i) we have correctly measured or identified all of the factors 
affecting our business or the extent of these factors likely impact, (ii) the 
available information with respect to these factors on which such analysis is 
based is complete or accurate, (iii) such analysis is correct or (iv) our 
strategy, which is based in part on this analysis, will be successful. All 
forward-looking statements in this report apply only as of the date of this 
report or as of the date they were made and, except as required by applicable 
law, we undertake no obligation to publicly update any forward-looking 
statement, whether as a result of new information, future developments or 
otherwise.
                                                                                
                                       4                                        
-------------------------------------------------------------------------------

                          Cyclerion Therapeutics, Inc.                          
                              Condensed Consolida                               
                               ted Balance Sheets                               
                 (In thousands except share and per share data)                 
                                  (Unaudited)                                   


                                                                             
                                                 March 31,     December 31,  
                                                   2023           2022       
ASSETS                                                                       
Current assets:                                                              
Cash and cash equivalents                        $   7,169        $  13,382  
Accounts receivable                                     96               96  
Prepaid expenses                                       567              805  
Other current assets                                   503              537  
Total current assets                                 8,335           14,820  
Operating lease right-of-use asset                   1,171            1,218  
Other assets                                         1,950            2,041  
Total assets                                     $  11,456        $  18,079  
LIABILITIES AND STOCKHOLDERS EQUITY                                          
Current liabilities:                                                         
Accounts payable                                 $   3,925        $   2,970  
Accrued research and development costs               1,605            2,275  
Accrued expenses and other current liabilities       2,001            2,382  
Total current liabilities                            7,531            7,627  
Commitments and contingencies (Note 6)                                       
Stockholders' equity                                                         
Common stock,                                                                
no                                                                           
par value,                                                                   
400,000,000                                                                  
shares authorized and                                                        
43,524,894                                                                   
issued and outstanding at March 31, 2023 and                                 
400,000,000                                                                  
shares authorized and                                                        
43,518,724                                                                   
issued and outstanding at December 31, 2022                                  
Paid-in capital                                    270,052          269,626  
Accumulated deficit                                      ( )              ( )
                                                   266,108          259,154  
Accumulated other comprehensive loss                     ( )              ( )
                                                        19               20  
Total stockholders' equity                           3,925           10,452  
Total liabilities and stockholders' equity       $  11,456        $  18,079  


  The accompanying notes are an integral part of these condensed consolidated   
                             financial statements.                              
                                                                                
                                       5                                        
-------------------------------------------------------------------------------

                          Cyclerion Therapeutics, Inc.                          
                         Condensed Consolidated Statem                          
                   ents of Operations and Comprehensive Loss                    
                      (In thousands except per share data)                      
                                  (Unaudited)                                   


                                                                       
                                                Three Months Ended     
                                                     March 31,         
                                                2023          2022     
Revenues:                                                              
Revenue from development agreement                                225  
Revenue from grants                                               486  
Total revenues                                                    711  
Cost and expenses:                                                     
Research and development                         3,773          9,743  
General and administrative                       3,269          3,952  
Total cost and expenses                          7,042         13,695  
Loss from operations                                 ( )            ( )
                                                 7,042         12,984  
Interest and other income (expenses), net           88              6  
Net loss                                      $      ( )     $      ( )
                                                 6,954         12,978  
Net loss per share:                                                    
Basic and diluted net loss per share          $      ( )     $      ( )
                                                  0.16           0.30  
Weighted average shares used in calculating:                           
Basic and diluted net loss per share            43,521         43,425  
                                                                       
Other comprehensive loss:                                              
Net loss                                      $      ( )     $      ( )
                                                 6,954         12,978  
Other comprehensive loss:                                              
Foreign currency translation adjustment gain         1                 
Comprehensive loss                            $      ( )     $      ( )
                                                 6,953         12,978  

                                                                                
  The accompanying notes are an integral part of these condensed consolidated   
                             financial statements.                              
                                                                                
                                       6                                        
-------------------------------------------------------------------------------

                          Cyclerion Therapeutics, Inc.                          
                          Condensed Consolidated State                          
                          ments of Stockholders Equity                          
                        (In thousands except share data)                        
                                  (Unaudited)                                   

                                                                                                                        
                                          Common            Paid-in     Accumulated     Accumulated          Total      
                                           Stock                                           other          Stockholders  
                                                                                        comprehensive                   
                                     Shares       Amount    capital      deficit            loss            equity      
Balance                             43,410,185     $       $ 263,345      $       ( )        $      ( )       $ 48,246  
at                                                                          215,076                23                   
December                                                                                                                
31, 2021                                                                                                                
Net                                                                               ( )                                ( )
loss                                                                         12,978                             12,978  
Issuance of common stock                38,175                                                                          
upon exercise of stock                                                                                                  
options, RSUs and employee                                                                                              
stock purchase plan                                                                                                     
Share-based compensation expense                               1,476                                             1,476  
related to issuance of stock                                                                                            
options and RSUs to employees and                                                                                       
employee stock purchase plan                                                                                            
Sharebased compensation                                          291                                               291  
expense related to                                                                                                      
issuance of stock options                                                                                               
to non-employees                                                                                                        
Foreign                                                                                             ( )              ( )
currency                                                                                            1                1  
translation                                                                                                             
adjustment                                                                                                              
Balance                             43,448,360     $       $ 265,112      $       ( )        $      ( )       $ 37,034  
at                                                                          228,054                24                   
March 31,                                                                                                               
2022                                                                                                                    


                                                                                
                                       7                                        
-------------------------------------------------------------------------------

                          Cyclerion Therapeutics, Inc.                          
            Condensed Consolidated Statements of Stockholders Equity            
                        (In thousands except share data)                        
                                  (Unaudited)                                   


                                                                                                                        
                                          Common            Paid-in     Accumulated     Accumulated          Total      
                                           Stock                                           other          Stockholders  
                                                                                        comprehensive                   
                                     Shares       Amount    capital      deficit            loss            equity      
Balance                             43,518,724     $       $ 269,626      $       ( )        $      ( )       $ 10,452  
at                                                                          259,154                20                   
December                                                                                                                
31, 2022                                                                                                                
Net                                                                               ( )                                ( )
loss                                                                          6,954                              6,954  
Issuance of common stock                 6,170                                                                          
upon exercise of stock                                                                                                  
options, RSUs and employee                                                                                              
stock purchase plan                                                                                                     
Share-based compensation expense                                 416                                               416  
related to issuance of stock                                                                                            
options and RSUs to employees and                                                                                       
employee stock purchase plan                                                                                            
Sharebased compensation                                           10                                                10  
expense related to                                                                                                      
issuance of stock options                                                                                               
to non-employees                                                                                                        
Foreign                                                                                             1                1  
currency                                                                                                                
translation                                                                                                             
adjustment                                                                                                              
Balance                             43,524,894     $       $ 270,052      $       ( )        $      ( )       $  3,925  
at                                                                          266,108                19                   
March 31,                                                                                                               
2023                                                                                                                    


  The accompanying notes are an integral part of these condensed consolidated   
                             financial statements.                              
                                                                                
                                       8                                        
-------------------------------------------------------------------------------

                          Cyclerion Therapeutics, Inc.                          
                             Condensed Consolidated                             
                            Statements of Cash Flows                            
                                 (In thousands)                                 
                                  (Unaudited)                                   


                                                                                                        
                                                                                 Three Months Ended     
                                                                                      March 31,         
                                                                                 2023          2022     
CASH FLOWS FROM OPERATING ACTIVITIES:                                                                   
Net loss                                                                       $      ( )     $      ( )
                                                                                  6,954         12,978  
Adjustments to reconcile net loss to net cash (used in) operating activities:                           
Depreciation and amortization                                                                       48  
Share-based compensation expense                                                    426          1,767  
Changes in operating assets and liabilities:                                                            
Accounts receivable                                                                                  ( )
                                                                                                   127  
Prepaid expenses                                                                    238              ( )
                                                                                                    79  
Other current assets                                                                 34              ( )
                                                                                                     9  
Operating lease assets                                                               47             46  
Other assets                                                                         91             91  
Accounts payable                                                                    955            691  
Accrued research and development costs                                                ( )            ( )
                                                                                    670          1,815  
Accrued expenses and other current liabilities                                        ( )            ( )
                                                                                    381            470  
Net cash (used in) operating activities                                               ( )            ( )
                                                                                  6,214         12,835  
Effect of exchange rate changes on cash and cash equivalents                          1              ( )
                                                                                                     1  
Net decrease in cash, cash equivalents and restricted cash                            ( )            ( )
                                                                                  6,213         12,836  
Cash, cash equivalents and restricted cash, beginning of period                  13,382         53,961  
Cash, cash equivalents and restricted cash, end of period                      $  7,169       $ 41,125  


  The accompanying notes are an integral part of these condensed consolidated   
                             financial statements.                              
                                                                                
                                       9                                        
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                          Cyclerion Therapeutics, Inc.                          
                      Notes to the Condensed Consolidated                       
                              Financial Statements                              
                                  (Unaudited)                                   
1. Nature of Business
Nature of Operations
Cyclerion Therapeutics, Inc. (Cyclerion, the Company or we) is a biopharmaceutic
al company on a mission to develop treatments for serious diseases. Our 
portfolio includes novel soluble guanylate cyclase ("sGC") stimulators that 
modulate a key node in a fundamental signaling network in both the central 
nervous system ("CNS") and the periphery. The nitric oxide ("NO") soluble 
guanylate cyclase ("sGC") cyclic guanosine monophosphate ("cGMP") signaling 
pathway is a fundamental mechanism that precisely controls key aspects of 
physiology throughout the body. The NO-sGC-cGMP pathway, regulates diverse and 
critical biological functions including mitochondrial function, neuronal 
function, inflammation, and hemodynamics. Although this pathway has been 
successfully targeted with several drugs in the periphery, this mechanism has 
yet to be fully leveraged therapeutically, particularly in the CNS, where 
impaired NO-sGC-cGMP signaling is believed to play an important role in the 
pathogenesis of many neurodegenerative and neuropsychiatric diseases.
Zagociguat is a clinical-stage CNS-penetrant sGC stimulator that has shown 
rapid improvement in cerebral blood flow, functional brain connectivity, brain 
response to visual stimulus, cognitive performance, and biomarkers associated 
mitochondrial function and inflammation in clinical studies. CY 3018 is a 
CNS-targeted sGC stimulator that preferentially localizes to the brain and has 
a pharmacology profile that suggests its potential for the treatment of 
neuropsychiatric diseases and disorders. Praliciguat is a systemic sGC 
stimulator that is licensed to Akebia Therapeutics, Inc. ("Akebia") and being 
advanced in rare kidney disease. Olinciguat is a clinical-stage vascular sGC 
stimulator that the Company intends to out-license for cardiovascular 
diseases. Cyclerion is actively evaluating the best combination of capital, 
capabilities, and transactions available to it to advance the development of 
zagociguat and its other clinical development candidates and to maximize 
shareholder value.
Cyclerion GmbH, a wholly owned subsidiary, was incorporated in Zug, 
Switzerland on May 3, 2019. The functional currency is the Swiss franc.

Cyclerion Securities Corporation, a wholly owned subsidiary, was incorporated 
in Massachusetts on November 15, 2019 and was granted securities corporation 
status in Massachusetts for the 2019 tax year. Cyclerion Securities 
Corporation has
no
employees.
Company Overview
The Companys mission is to develop treatments for serious CNS diseases.
Zagociguat is an orally administered CNS-penetrant sGC stimulator. As an sGC 
stimulator, zagociguat amplifies endogenous NO signaling by acting as a 
positive allosteric modulator to sensitize the sGC enzyme to NO and increase 
the production of cGMP, and thereby amplify endogenous NO signaling. By 
compensating for deficient NO-sGC-cGMP signaling zagociguat may have broad 
therapeutic potential as a treatment for people with serious diseases.
On January 13, 2020, we announced positive results from our Phase 1 
first-in-human study that provided the foundation for continued development of 
zagociguat. The results from this study indicate that zagociguat was well 
tolerated. Pharmacokinetic data, obtained from both blood and cerebral spinal 
fluid ("CSF"), support once-daily dosing, with or without food, and 
demonstrated zagociguat penetration of the blood-brain-barrier with CSF 
concentrations expected to be pharmacologically active.
On October 14, 2020, we announced positive topline results from our zagociguat 
Phase 1 translational pharmacology study in healthy elderly participants. 
Treatment with zagociguat for 15-days in this 24-subject study confirmed and 
extended results seen in the earlier first-in-human Phase 1 study: once daily 
oral treatment demonstrated blood-brain barrier penetration with expected CNS 
exposure and target engagement. Results also showed significant improvements 
in neurophysiological and objective performance measures as well as decreases 
in
                                                                                
                                       10                                       
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inflammatory biomarkers associated with aging and neurodegenerative diseases. 
Zagociguat was safe and generally well tolerated in the study. These results, 
together with nonclinical data, supported the continued development of 
zagociguat as a potential new medicine for serious diseases involving the CNS.

On June 10, 2022, we announced positive topline clinical data for zagociguat 
in our signal-seeking clinical study for the potential treatment of 
Mitochondrial Encephalomyopathy, Lactic Acidosis and Stroke-like episodes 
("MELAS"). In this open-label, single-arm study of the oral, once-daily sGC 
stimulator in eight adults aged 18 or older with MELAS, improvements were seen 
across a range of endpoints reflecting multiple domains of disease activity, 
including mitochondrial disease-associated biomarkers such as lactate and 
GDF-15, a broad panel of inflammatory biomarkers, cerebral blood flow, and 
functional connectivity between neural networks. These positive effects after 
29 days of dosing were supported by correlations among several endpoints with 
each other and with zagociguat plasma concentrations. Zagociguat was well 
tolerated with no serious or severe adverse events and no events leading to 
discontinuation. Pharmacokinetics were consistent with the Phase 1 studies in 
healthy volunteers. The positive data from this study support the potential of 
zagociguat to provide therapeutic benefit to people living with mitochondrial 
diseases, including MELAS.
On July 28, 2022, we announced positive topline data from our signal-seeking 
clinical study of zagociguat for the potential treatment of Cognitive 
Impairment Associated with Schizophrenia ("CIAS"). Data from the 14-day, 
double blind, randomized, placebo-controlled, multiple-ascending-dose study in 
48 adults aged 18-50 with stable schizophrenia on a stable, single atypical 
antipsychotic regimen demonstrated that once-daily zagociguat was safe and 
well tolerated, with no reports of serious adverse events, severe adverse 
events, or treatment discontinuation due to adverse events. We further 
announced that study data demonstrated a strong effect on cognitive 
performance after two weeks of 15mg once-daily dosing and that positive 
movements on inflammatory biomarkers were also observed. These signals on 
exploratory endpoints are consistent with pro-cognitive and anti-inflammatory 
effects of zagociguat observed in preclinical studies and prior clinical 
trials and support the further development of oral, once-daily zagociguat.

In October 2022, the WHO International Nonproprietary Names committee and the 
United States Adopted Name council selected zagociguat as a nonproprietary 
name for CY6463.
On March 22, 2023, we announced that given the significant capital and 
capabilities necessary to ensure that the MELAS Phase 2b study is executed 
efficiently and with the highest quality, and the currently unfavorable 
capital market conditions, we are actively evaluating the best combination of 
capital, capabilities, and transactions available to us to advance the 
development of zagociguat and our other clinical development candidates and to 
maximize shareholder value.
On March 31, 2023, we entered into a stock purchase agreement with the 
Company's Chief Executive Officer (the "CEO") pursuant to which the CEO will 
make an equity investment in the Company of $
5
million in cash for common stock or nonvoting convertible preferred stock of 
the Company, the purchase price, consistent with Nasdaq rules, to be at or 
above the market price at the time of signing that agreement. The closing of 
the equity investment will take place six business days after the signing of 
the Asset Purchase Agreement (as defined below), or May 11, 2023.
On May 11, 2023, the Company entered into an Asset Purchase Agreement (the 
"Asset Purchase Agreement) with an investor group which includes Peter Hecht, 
the Company's Chief Executive Officer ("CEO"), JW Celtics Investment Corp. 
(Buyer Parent) and JW Cycler Inc. (Buyer). Pursuant to the terms of the Asset 
Purchase Agreement and subject to the approval by the Company's shareholders, 
the Company will receive proceeds of $
8
million as cash consideration, reimbursement for certain operating expenses 
related to zagociguat and CY3018 for the period between signing and closing of 
the transaction and
10
% of all of Buyer Parent's outstanding equity securities upon the successful 
closing of the transaction.
On
May 11, 2023, we announced topline data from our signal- seeking clinical 
study of zagociguat for the potential treatment of Alzheimer's disease with 
vascular pathology ("ADv"), which study was supported in part by a $2 million 
grant from the Alzheimer's Association's Part the Cloud-Gates Partnership 
Grant Program (the "PTC Grant") . This exploratory, randomized, placebo-controll
ed, study of oral once-daily zagociguat was designed to evaluate safety, 
tolerability, and pharmacokinetics as well as explore the impact of zagociguat 
on biomarkers and
                                                                                
                                       11                                       
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cognitive
performance over a twelve-week dosing period. The total number of enrolled 
participants was capped at 12 participants due to challenges associated with 
enrollment. Data from this study show that the safety and tolerability of 
profile once-daily zagociguat was consistent with prior studies. Given the 
small number of participants we are unable to draw any conclusions from the 
data generated in the study.
CY3018 is a CNS-targeted sGC stimulator in preclinical development that 
preferentially localizes to the brain and has a pharmacology profile that 
suggests its potential for the treatment of neuropsychiatric diseases and 
disorders.
Praliciguat is an orally administered, once-daily systemic sGC stimulator. On 
June 3, 2021, we entered into a license agreement with Akebia relating to the 
exclusive worldwide license to Akebia of our rights to the development, 
manufacture, medical affairs and commercialization of pharmaceutical products 
containing praliciguat and other related products and forms thereof enumerated 
in such agreement. Cyclerion is eligible to receive up to $
585
million in total potential future development, regulatory, and commercialization
 milestone payments. Cyclerion is also eligible to receive tiered, sales-based 
royalties ranging from single-digit to high-teen percentages.
Olinciguat
is an orally administered, once-daily, vascular sGC stimulator that was 
evaluated in a Phase 2 study of participants with sickle cell disease. We 
released topline results from this study in October 2020. We intend to 
out-license olinciguat to an entity with strong cardiovascular and/or 
cardiopulmonary capabilities.
At-the-Market Offering
On July 24, 2020, the Company filed a Registration Statement on Form S-3 (the 
"Shelf") with the Securities and Exchange Commission (the SEC) in relation to 
the registration of common stock, preferred stock, debt securities, warrants 
and units of any combination thereof for an aggregate initial offering price 
not to exceed $
150.0
million. The Shelf was declared effective as of July 31, 2020. On September 3, 
2020, the Company entered into a Sales Agreement (the Sales Agreement) with 
Jefferies LLC (Jefferies) with respect to an at-the-market offering (the ATM 
Offering) under the Shelf. Under the ATM Offering, the Company may offer and 
sell, from time to time at its sole discretion, shares of its common stock, 
having an aggregate offering price of up to $
50.0
million through Jefferies as its sales agent. The Company will pay Jefferies 
cash commissions of
3.0
percent of the gross proceeds of sales of common stock under the Sales 
Agreement. The Company has sold
3,353,059
shares of its common stock for net proceeds of $
12.5
million under the ATM Offering since entering into the Sales Agreement.
No
shares of common stock have been issued or sold under the ATM Offering during 
the
three months ended March 31, 2023.
Basis of Presentation
The condensed consolidated financial statements and the related disclosures 
are unaudited and have been prepared in accordance with accounting principles 
generally accepted in the U.S. Additionally, certain information and footnote 
disclosures normally included in the Companys annual financial statements have 
been condensed or omitted. Accordingly, these interim condensed consolidated 
financial statements should be read in conjunction with the consolidated 
financial statements and notes thereto contained in the Companys Annual Report 
on Form 10-K for the fiscal year ended December 31, 2022, which was filed with 
the Securities and Exchange Commission on March 22, 2023.
In the opinion of management, the unaudited interim condensed consolidated 
financial statements reflect all normal recurring adjustments considered 
necessary for a fair presentation of the Companys financial position and the 
results of its operations for the interim periods presented. The results of 
operations for the three months ended March 31, 2023 and 2022 are not 
necessarily indicative of the results that may be expected for the full year 
or any other subsequent interim period.
                                                                                
                                       12                                       
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The condensed consolidated financial statements include the financial 
statements of the Company and its wholly owned subsidiaries, Cyclerion GmbH, 
and Cyclerion Securities Corporation. All significant intercompany accounts 
and transactions have been eliminated in the preparation of the accompanying 
condensed consolidated financial statements.
Going Concern
At each reporting period, in accordance with Accounting Standards Codification 
("ASC") 205-40, Going Concern, the Company evaluates whether there are 
conditions or events that raise substantial doubt about the Companys ability 
to continue as a going concern within one year after the date that the 
financial statements are issued. The Companys evaluation entails analyzing 
prospective operating budgets and forecasts for expectations of the Companys 
cash needs and comparing those needs to the current cash and cash equivalent 
balances. The Company is required to make certain additional disclosures if it 
concludes substantial doubt exists and it is not alleviated by the Companys 
plans or when its plans alleviate substantial doubt about the Companys ability 
to continue as a going concern.
This evaluation initially does not take into consideration the potential 
mitigating effect of managements plans that have not been fully implemented as 
of the date the financial statements are issued. When substantial doubt exists 
under this methodology, management evaluates whether the mitigating effect of 
its plans sufficiently alleviates substantial doubt about the Companys ability 
to continue as a going concern. The mitigating effect of managements plans, 
however, is only considered if both (1) it is probable that the plans will be 
effectively implemented within one year after the date that the financial 
statements are issued, and (2) it is probable that the plans, when 
implemented, will mitigate the relevant conditions or events that raise 
substantial doubt about the entitys ability to continue as a going concern 
within one year after the date that these consolidated financial statements 
are issued. In performing its analysis, management excluded certain elements 
of its operating plan that cannot be considered probable. Under ASC 205-40, 
the future receipt of potential funding from future partnerships, equity or 
debt issuances and the potential milestones from the Akebia agreement cannot 
be considered probable at this time because these plans are not entirely 
within the Companys control and/or have not been approved by the Board of 
Directors as of the date of these consolidated financial statements.
The Companys expectation to generate negative operating cash flows in the 
future and the need for additional funding to support its planned operations, 
raise substantial doubt regarding the Companys ability to continue as a going 
concern for a period of one year after the date that these consolidated 
financial statements are issued. Management's plans to alleviate the 
conditions that raise substantial doubt include reduced spending, and the 
pursuit of additional capital. Management has concluded the likelihood that 
its plan to successfully obtain sufficient funding from one or more of these 
sources, or adequately reduce expenditures, while reasonably possible, is less 
than probable. Accordingly, the Company has concluded that substantial doubt 
exists about the Company's ability to continue as a going concern for a period 
of at least 12 months from the date of issuance of these consolidated 
financial statements.
The accompanying financial statements have been prepared on a going concern 
basis, which contemplates the realization of assets and satisfaction of 
liabilities in the ordinary course of business. The financial statements do 
not include any adjustments relating to the recoverability and classification 
of recorded asset amounts or the amounts and classification of liabilities 
that might result from the outcome of the uncertainties described above.

Notice of Delisting or Failure to Satisfy a Continued Listing Rule or Standard
On June 1, 2022, the Company received a notice from the Nasdaq Stock Market 
("Nasdaq") notifying the Company that, for the last
30
consecutive business days, the closing bid price for the Company's common 
stock listed on Nasdaq has been below the minimum $
1.00
per share required for continued listing on the Nasdaq Global Select Market 
pursuant to Nasdaq Listing Rule 5450(a)(1) (the "Bid Price Requirement").
In accordance with Nasdaq Listing Rule 5810(c)(3)(A), the Company was provided 
a period of
180
calendar days, or until November 28, 2022, to regain compliance with the Bid 
Price Requirement. The Company did not regain compliance with the Bid Price 
Requirement by the initial compliance date. On November 29, 2022, Nasdaq 
notified the Company that it is eligible for an additional
180
calendar
day period, or until May 29, 2023 (the "Extended Compliance Date"), to regain 
compliance with the Bid Price Requirement. Nasdaqs determination was
                                                                                
                                       13                                       
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based
on the Company meeting the continued listing requirement for market value of 
publicly held shares and all other applicable requirements for initial listing 
on the Nasdaq Capital Market with the exception of the Bid Price Requirement, 
and the Companys written notice of its intention to cure the deficiency during 
the second compliance period by effecting a reverse stock split, if necessary. 
Effective November 25, 2022, the Company transferred its listing of the 
Companys common stock from the Nasdaq Global Market to the Nasdaq Capital 
Market, a continuous trading market that operates in substantially the same 
manner as the Nasdaq Global Market. The Companys common stock continues to 
trade under the symbol CYCN.
To date, the Company has not regained compliance with the Bid Price 
Requirement. If at any time before May 29, 2023, the bid price of the 
Company's common stock closes at a $
1.00
per share or more for a minimum of
10
consecutive business days, Nasdaq will provide written notification to the 
Company that it has regained compliance with the Bid Price Requirement. If the 
Company does not regain compliance with the Bid Price Requirement by the end 
of the second compliance period, the Company's stock will be subject to 
delisting.
On April 3, 2023, the Company filed with the SEC a definitive proxy statement 
for its annual meeting of stockholders to be held on May 15, 2023, at which 
meeting the Company is seeking stockholder approval of a reverse stock split 
with the primary intent of increasing the price of its common stock to meet 
the price criteria for continued listing on Nasdaq. The Company intends to 
monitor the closing bid price of its common stock and may, if appropriate, 
consider available options to regain compliance with the Bid Price 
Requirement, including initiating a reverse stock split. However, there can be 
no assurance on how stockholders will vote on such proposal or that the 
Company will be able to regain compliance with the Bid Price Requirement or 
will otherwise be in compliance with other Nasdaq Listing Rules.
2. Summary of Significant Accounting Policies
The accounting policies of the Company are set forth in Note 2.
Summary of Significant Accounting Policies
to the consolidated financial statements contained in the Companys Annual 
Report on Form 10-K for the fiscal year ended December 31, 2022.
Use of Estimates
The preparation of consolidated financial statements in accordance with U.S. 
generally accepted accounting principles ("GAAP") requires the Companys 
management to make estimates and judgments that may affect the reported 
amounts of assets and liabilities and disclosure of contingent assets and 
liabilities at the date of the consolidated financial statements, and the 
amounts of expenses during the reported periods. On an ongoing basis, the 
Companys management evaluates its estimates, judgments, and methodologies. 
Significant estimates and assumptions in the consolidated financial statements 
include those related to revenue, impairment of long-lived assets, valuation 
procedures for right-of-use ("ROU") assets and operating lease liabilities, 
income taxes, including the valuation allowance for deferred tax assets, 
research and development expenses, contingencies, share-based compensation and 
going concern. The Company bases its estimates on historical experience and on 
various other assumptions that are believed to be reasonable, the results of 
which form the basis for making judgments about the carrying values of assets 
and liabilities. Actual results may differ materially from these estimates 
under different assumptions or conditions. Changes in estimates are reflected 
in reported results in the period in which they become known.
New Accounting Pronouncements
From time to time, new accounting pronouncements are issued by the Financial 
Accounting Standards Board (FASB) or other standard setting bodies that are 
adopted by the Company as of the specified effective date. Except as discussed 
elsewhere in the notes to the consolidated financial statements, the Company 
did not adopt any new accounting pronouncements during the three months ended 
March 31, 2023 that had a material effect on its condensed consolidated 
financial statements.
In June 2016 the FASB issued ASU 2016-13, Financial Instruments-Credit Losses. 
This standard requires entities to measure all expected credit losses for 
financial assets held at the reporting date based on historical experience, 
current conditions, and reasonable and supportable forecasts. As a smaller 
reporting company, ASU 2016-13 will become effective for the Company for 
fiscal years beginning after December 15, 2022, and early
                                                                                
                                       14                                       
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adoption is permitted. The Company adopted ASU 2016-13 in the first quarter of 
2023, and the adoption of this standard did not have any impact on the 
Company's financial position or results of operations
No other accounting standards known by the Company to be applicable to it that 
have been issued by the FASB or other standard-setting bodies and that do not 
require adoption until a future date are expected to have a material impact on 
the Companys condensed consolidated financial statements upon adoption.
3. Fair Value of Financial Instruments
The Companys cash equivalents are generally classified within Level 1 of the 
fair value hierarchy. The following tables present information about the 
Companys financial assets measured at fair value on a recurring basis and 
indicate the level of the fair value hierarchy used to determine such fair 
values as of
March 31, 2023 and December 31, 2022 (in thousands):


                                                                               
                          Fair Value Measurements as of March 31, 2023:        
                     Level 1         Level 2          Level 3          Total   
Cash equivalents:                                                              
Money market funds   $ 6,079          $                $               $ 6,079 
Cash equivalents     $ 6,079          $                $               $ 6,079 



                                                                                  
                          Fair Value Measurements as of December 31, 2022:        
                     Level 1           Level 2          Level 3           Total   
Cash equivalents:                                                                 
Money market funds   $ 12,357           $                $               $ 12,357 
Cash equivalents     $ 12,357           $                $               $ 12,357 


During the three months ended March 31, 2023 and 2022, there were no transfers 
between levels. The fair value of the Companys cash equivalents, consisting of 
money market funds, is based on quoted market prices in active markets with no 
valuation adjustment.
The Company believes the carrying amounts of its prepaid expenses and other 
current assets, restricted cash, accounts receivable, accounts payable, and 
accrued expenses approximate their fair value due to the short-term nature of 
these amounts.
4. Property and Equipment
Property and equipment, net consisted of the following (in thousands):


                                                                              
                                                  March 31,     December 31,  
                                                    2023           2022       
Software                                              2,174         $  2,174  
Computer equipment                                                         7  
Leasehold improvements                                                        
Property and equipment, gross                         2,174            2,181  
Less: accumulated depreciation and amortization           ( )              ( )
                                                      2,174            2,181  
Property and equipment, net                         $               $         


As of March 31, 2023, and December 31, 2022, the Companys property and 
equipment was primarily located in Boston, Massachusetts.
During the three months ended March 31, 2023
, the Company did
not
record depreciation and amortization expenses. The company recorded 
approximately $
0.1
million of depreciation and amortization expenses for the
three months ended March 31, 2022
.
                                                                                
                                       15                                       
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5. Accrued Expenses and Other Current Liabilities
Accrued expenses and other current liabilities consisted of the following (in 
thousands):


                                                                           
                                                 March 31,    December 31, 
                                                   2023          2022      
Accrued incentive compensation                     $   362        $    238 
Salaries                                               248             246 
Accrued vacation                                       186             186 
Professional fees                                      924             835 
Accrued severance and benefit costs                    141             809 
Other                                                  140              68 
Accrued expenses and other current liabilities     $ 2,001        $  2,382 


6. Commitments and Contingencies
Other Funding Commitments
In the normal course of business, the Company enters into contracts with 
clinical research organizations and other third parties for clinical and 
preclinical research studies and other services and products for operating 
purposes. These contracts are generally cancellable, with notice, at the 
Companys option and do not have any significant cancellation penalties.
Guarantees
On September 6, 2018, Cyclerion was incorporated in Massachusetts and its 
officers and directors are indemnified for certain events or occurrences while 
they are serving in such capacity.
The Company enters into certain agreements with other parties in the ordinary 
course of business that contain indemnification provisions. These typically 
include agreements with directors and officers, business partners, 
contractors, clinical sites and customers. Under these provisions, the Company 
generally indemnifies and holds harmless the indemnified party for losses 
suffered or incurred by the indemnified party as a result of the Companys 
activities. These indemnification provisions generally survive termination of 
the underlying agreements. The maximum potential amount of future payments the 
Company could be required to make under these indemnification provisions is 
unlimited. However, to date the Company has not incurred material costs to 
defend lawsuits or settle claims related to these indemnification provisions. 
As a result, the estimated fair value of these obligations is minimal. 
Accordingly, the Company did not have any liabilities recorded for these 
obligations as of March 31, 2023 and December 31, 2022
.
7. Leases

In May 2021, the Company signed a
12-month
membership agreement to lease space with WeWork at 501 Boylston Street, 
Boston, Massachusetts, commencing on August 1, 2021. The agreement was 
extended for six months on August 1, 2022. The
12-month
agreement and
6-month
extension are accounted for as short-term leases. The Company recorded a de 
minimis amount and approximately $
0.1
million, in lease expense associated with the membership agreement during the 
three months ended March 31, 2023 and 2022, respectively.
On September 15, 2020, the Company entered into a Sublease Termination 
Agreement (the "Sublease Termination Agreement") to terminate its sublease of

15,700
rentable square feet of its leased premises under the Head Lease. Under the 
terms of the Sublease Termination Agreement, the subtenant was relieved of its 
obligation to provide future cash rental payments to the Company. The 
agreements requiring the former subtenant to provide licensed rooms and 
services to the Company free of charge through the original sublease term 
survived the sublease termination. The Company gained access to the licensed 
rooms and services beginning in the third quarter of 2021. The letter of 
credit security deposit related to the sublease was released.
The
Company determined that the Sublease Termination Agreement constituted a 
non-monetary exchange under ASC 845 Nonmonetary Transactions ("ASC 845") 
where, in return for the free rooms and the services, the
                                                                                
                                       16                                       
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Company
agreed to terminate its rights and obligations under the sublease agreement. 
In accordance with ASC 845, the Company determined that the accounting for the 
transaction should be based on the fair value of assets or services involved. 
The Company estimated the fair value of the rooms and services to be 
approximately $
1.5
million and $
2.9
million, respectively.
The Company determined that the licensed rooms represent a lease under ASC 
Topic 842, Leases. The Company obtained control of the rooms in the third 
quarter of 2021 and the prepaid rooms balance of approximately $
1.4
million was reclassified from other assets to a ROU asset. The related lease 
expense is recognized on a straight-line basis over the lease term of
8.88
years. The Company recorded $
0.1
million and $
0.1
million of lease expense during the
three months ended March 31, 2023 and 2022, respectively. The Company 
determined that the licensed services represent a non-lease component, which 
is recognized separately from the lease component for this asset class. The 
expense related to the licensed services is recognized on a straight-line 
basis over the period the services are received. The Company recorded $
0.1
million and $
0.1
million for the
three months ended March 31, 2023 and 2022
, respectively. Both the lease expense and services expense are recognized as 
a component of research and development costs in the condensed consolidated 
statements of operations and comprehensive loss.
8. Share-based Compensation Plans
In 2019, Cyclerion adopted share-based compensation plans. Specifically, 
Cyclerion adopted the 2019 Employee Stock Purchase Plan (2019 ESPP) and the 
2019 Equity Incentive Plan (2019 Equity Plan). Under the 2019 ESPP, eligible 
employees may use payroll deductions to purchase shares of stock in offerings 
under the plan, and thereby acquire an interest in the future of the Company. 
The 2019 Equity Plan provides for stock options and restricted stock units 
(RSUs).
Cyclerion mirrored two of Ironwood Pharmaceuticals, Incs ("Ironwood") existing 
plans, the Amended and Restated 2005 Stock Incentive Plan (2005 Equity Plan) 
and the Amended and Restated 2010 Employee, Director and Consultant Equity 
Incentive Plan (2010 Equity Plan"). These mirror plans were adopted to 
facilitate the exchange of Ironwood equity awards for Cyclerion equity awards 
upon the tax-free spin-off of Ironwood's sGC business (the "Separation") as 
part of the equity conversion. As a result of the Separation and in accordance 
with the Employee Matters Agreement between Ironwood and Cyclerion entered 
into as part of the Separation, employees of both companies retained their 
existing Ironwood vested options and received a pro-rata share of Cyclerion 
options, regardless of which company employed them post-Separation. For 
employees that were ultimately employed by Cyclerion, unvested Ironwood 
options and RSUs were converted to unvested Cyclerion options and RSUs.
The following table provides share-based compensation reflected in the 
Companys condensed consolidated statements of operations and comprehensive 
loss for the
three months ended March 31, 2023 and 2022 (in thousands):


                                                    
                              Three Months Ended    
                                   March 31,        
                             2023            2022   
Research and development     $ 229          $   830 
General and administrative     197              937 
                             $ 426          $ 1,767 


                                                                                
                                       17                                       
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A summary of stock option activity for the
three months ended March 31, 2023, is as follows:


                                                                                            
                                                                  Weighted                  
                                                     Weighted      Average        Average   
                                                     Average      Remaining      Intrinsic  
                                       Number        Exercise    Contractual     Value (in  
                                      of Options      Price      Term (Years)    thousands) 
Outstanding as of December 31, 2022    7,311,893      $  9.23             5.8            20 
Granted                                                  0.00                               
Exercised                                                0.00                               
Cancelled or forfeited                         ( )      14.29                               
                                         600,887                                            
Outstanding as of March 31, 2023       6,711,006      $  8.77             6.1       $     0 
Exercisable at March 31, 2023          4,867,431      $ 11.30             5.2       $     0 

As of March 31, 2023, the unrecognized share-based compensation expense, net 
of estimated forfeitures, related to all unvested time-based stock options 
held by the Companys employees is $
1.8
million and the weighted average period over which that expense is expected to 
be recognized is
3.2
years.
A summary of RSU activity for the
three months ended March 31, 2023 is as follows:


                                                                  
                                                 Weighted Average 
                                    Number         Grant Date     
                                   of Shares       Fair Value     
Unvested as of December 31, 2022     815,587           $     0.77 
Granted                                                           
Vested                                     ( )              14.20 
                                       6,170                      
Forfeited                                                         
Unvested as of March 31, 2023        809,417           $     0.66 


As of March 31, 2023, the unrecognized share-based compensation expense, net 
of estimated forfeitures, related to all unvested restricted stock units by 
the Companys employees is $
0.3
million and the weighted-average period over which that expense is expected to 
be recognized is
0.52
years.

The Company has granted to certain employees stock options containing market 
conditions that vest upon the achievement of specified price targets of the 
Companys share price for a period through December 31, 2024. Vesting is 
measured based upon the average closing price of the Companys share price for 
any
thirty
consecutive trading days, subject to certain service requirements. Stock 
compensation cost is expensed on a straight-line basis over the derived 
service period for each stock price target within the award, ranging from 
approximately
4.0
to
4.6
years. The Company accelerates expense when a stock price target is achieved 
prior to the derived service period. As of
March 31, 2023
there were
300,000
outstanding stock options containing market conditions with a weighted average 
exercise price of $
2.01
. As of
March 31, 2023
there was $
0.1
million of unrecognized compensation costs related to stock options containing 
market conditions, which is expected to be recognized over a weighted-average 
period of
1
year.
                                                                                
                                       18                                       
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9. Loss per share
Basic and diluted net loss per common share is computed by dividing net loss 
by the weighted average number of common shares outstanding during the period 
as follows:


                                                                                                                           
                                                                                                    Three Months Ended     
                                                                                                         March 31,         
                                                                                                    2023          2022     
Numerator:                                                                                                                 
Net loss (in thousands)                                                                           $      ( )     $      ( )
                                                                                                     6,954         12,978  
                                                                                                                           
Denominator:                                                                                                               
Weighted average shares used in calculating net loss per share  basic and diluted (in thousands)    43,521         43,425  
Net loss per share  basic and diluted                                                             $      ( )     $      ( )
                                                                                                      0.16           0.30  

We exclude shares of common stock related to stock options and RSUs from the 
calculation of diluted net loss per share since the inclusion of such shares 
would be anti-dilutive.
The following table sets forth potential shares that were considered 
anti-dilutive for the
three months ended March 31, 2023 and 2022:


                                         
                  Three Months Ended     
                       March 31,         
                  2023          2022     
Stock Options  $ 6,711,006   $ 8,270,398 
RSUs               809,417        54,629 
               $ 7,520,423   $ 8,325,027 


10. Defined Contribution Plan
Subsequent to the Separation, the Company adopted a defined contribution
401(k) Savings Plan
similar to the plan in place at Ironwood. The plan assets under the Ironwood 
defined contribution 401(k) Savings Plan were transferred to the Company's 
plan.
Subject to certain IRS limits, eligible employees may elect to contribute from
1
% to
100
% of their compensation. The Company's contributions to the plan are at the 
sole discretion of the board of directors. Currently, the Company provides a 
matching contribution of
75
% of the employees contributions, up to $
6,000
annually.
Included in compensation expense is approximately $
0.1
million and $
0.2
million related to the defined contribution 401(k) Savings Plan for the
three months ended March 31, 2023 and 2022
, respectively.
11. Workforce Reduction
2022 Workforce Reduction
On October 6, 2022, the Company began a reduction of its current workforce by
thirteen
(13) full-time employees to align its resources with its current priorities of 
focusing on a mitochondrial disease-focused strategy. The workforce reduction 
was completed in the fourth quarter of 2022.
The Company recorded total costs related to the 2022 Workforce Reduction of 
approximately $
1.3
million, including a de minimis amount of stock-based compensation from the 
modification of certain share-based equity awards.

The following table summarizes the accrued liabilities activity recorded in 
connection with the reduction in workforce for the
three months ended March 31, 2023 (in thousands):

                                                                                
                                       19                                       
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                             Amounts        Charges    Amount    Adjustments     Amounts    
                           accrued at                  paid                     accrued at  
                           December 31,                                         March 31,   
                              2022                                                2023      
2022 workforce reduction       $      ( )    $          $ 668       $              $     ( )
                                    809                                                141  
Total                          $      ( )    $          $ 668       $              $     ( )
                                    809                                                141  



12. License Agreement

Akebia License Agreement
On June 3, 2021, the Company and Akebia entered into a License Agreement (the 
Akebia License Agreement) relating to the exclusive worldwide license by the 
Company to Akebia of our rights to the development, manufacture, medical 
affairs and commercialization of pharmaceutical products containing the 
pharmaceutical compound known as praliciguat and other related products and 
forms thereof enumerated in the License Agreement (collectively, the 
Products). Pursuant to the Akebia License Agreement, Akebia will be 
responsible for all future research, development, regulatory, and 
commercialization activities for the Products.
Akebia paid a $
3.0
million up-front payment to the Company upon signing of the Akebia License 
Agreement and the Company is eligible to receive additional milestone cash 
payments of up to $
12.0
million upon initiation of a Phase 2 clinical trial. Further milestone cash 
payments by Akebia are scheduled in the Akebia License Agreement based on the 
initiation of Phase 3 clinical trials in the U.S. for Products for first and 
second indication, for FDA approvals, for approvals in certain other major 
markets, and for certain sales milestones. In addition to these cash milestone 
payments, Akebia will pay the Company tiered royalty payments on net sales in 
certain major markets at percentages ranging from the mid-single digits to the 
high-teens, subject to certain reductions and offsets.
Pursuant to the Akebia License Agreement, the Company determined the Akebia 
License Agreement represents a service arrangement under the scope of ASC 606. 
Given the reversion of the rights under the Akebia License Agreement 
represents a penalty in substance for a termination by Akebia, the contract 
term would be the stated term of the Akebia License Agreement.
The Company determined that the grant of license to our patents and 
trademarks, know how transfer, the assignment of regulatory submissions and 
trademarks and additional knowledge transfer assistance obligations represent 
a single promise and performance obligation to be transferred to Akebia over 
time due to the nature of the promises in the contract. The provision of 
development materials on hand was identified as a separate performance 
obligation. However, it is immaterial in the context of the contract as the 
development materials are low value and do not have an alternative use to the 
Company.
The consideration related to sales-based milestone payments, including 
royalties, will be recognized when the related sales occur as these amounts 
have been determined to relate predominantly to the license. The Company will 
re-evaluate the probability of achievement of the milestones and any related 
constraints each reporting period.
Akebia Supply Agreement
On August 3, 2021, the Company and Akebia entered into a Supply Agreement (the 
Supply Agreement) relating to the manufacturing by the Company of the Initial 
Supply of the Drug Product and placebo ("Initial Supply") for Akebia's use 
pursuant to the Akebia License Agreement. Akebia will pay the Company for the 
manufacturing costs at mutually agreed upon rates.
The Company determined the Supply Agreement has stand-alone value under the 
scope of ASC 606 and should not be combined with the Akebia License Agreement. 
Given that the Supply Agreement can be terminated at any time without cause 
with 30 days notice, the Company deemed the Supply Agreement to be a 
month-to-month contract. The manufacturing of the Initial Supply by the 
Company represents a single performance obligation and consideration related 
to the manufacturing costs will be recognized over time as costs are incurred. 
The Company recorded approximately $
0.2
million as revenue from the Supply Agreement in the three months ended March 
31, 2022. There was
no
revenue recognized as part of the Supply Agreement in the three months ended 
March 31, 2023.
                                                                                
                                       20                                       
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13. Grant Revenue
In August 2021, the Company was approved to receive funding from the PTC Grant
for the Phase 2 study of CNS sGC stimulation in AD with vascular features. The 
granting period was July 1, 2021, to December 31, 2022, and the Company 
received an award of $
2
million.
The Company determined that this transaction is non-reciprocal as there is not 
considered to be a commensurate value exchanged with the Alzheimer's 
Association as the funding provider. Where commensurate value is not exchanged 
for goods and services provided, a recipient assesses whether the grant is 
conditional or unconditional. The Company considered all conditions and 
barriers associated with this grant and determined the grant is conditional 
and revenue will be recognized upon achieving certain milestones and incurring 
internal costs specifically covered by this grant. Under ASC 958-605, revenues 
will be recognized as the Company incurs expenses related to the PTC Grant.
The Company has incurred
no
costs associated with the grant for the three months ended March 31, 2023, 
compared to approximately $
0.5
million of expenses associated with the grant for the three months ended March 
31, 2022. The Company had a deferred revenue balance of approximately $
0.1
million related to advance billings as of March 31, 2023
.
14. Subsequent Events
On May 11, 2023, the Company entered into the Asset Purchase Agreement with an 
investor group which includes the Company's CEO, Buyer Parent and Buyer. 
Pursuant to the terms of the Asset Purchase Agreement and subject to the 
approval by the Company's shareholders, the Company will receive proceeds of $

8
million as cash consideration, reimbursement for certain operating expenses 
related to zagociguat and CY3018 for the period between signing and closing of 
the transaction and
10
% of all of Buyer Parent's outstanding equity securities upon the successful 
closing of the transaction. The Company will also receive net proceeds of $
5
million pursuant to a stock purchase agreement with the Company's CEO, which 
will take place six business days after the signing of the Asset Purchase 
Agreement.
                                                                                
                                       21                                       
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Item 2.
Managements Discussion and Analysis of
Financial Condition and Results of Operations
Forward-Looking Information
The following discussion of our financial condition and results of operations 
should be read in conjunction with the unaudited condensed consolidated 
financial statements and the corresponding notes included in this Quarterly 
Report on Form 10-Q, as well as the audited condensed consolidated financial 
statements and notes thereto included in our Annual Report on Form 10-K for 
the fiscal year ended December 31, 2022. This discussion contains 
forward-looking statements that involve significant risks and uncertainties. 
As a result of many factors, such as those referenced or set forth under 
Cautionary Note Regarding Forward-Looking Statements and Risk Factors in Part 
II, Item 1A of this Quarterly Report on Form 10-Q, our actual results may 
differ materially from those anticipated in these forward-looking statements.

Overview
We are a biopharmaceutical company on a mission to develop treatments for 
serious diseases. Zagociguat is a pioneering CNS-penetrant sGC stimulator that 
has shown rapid improvements across a range of endpoints reflecting multiple 
domains of disease activity, including mitochondrial disease-associated 
biomarkers. sGC stimulators are small molecules that act synergistically with 
NO as positive allosteric modulators of sGC to boost production of cGMP. cGMP 
is a key second messenger that, when produced by sGC, regulates diverse and 
critical biological functions such as mitochondrial function, neuronal 
function, inflammation, and vascular dynamics.
We operate in one reportable business segmenthuman therapeutics.
Financial Overview
Research and Development Expense.
Research and development expenses are incurred in connection with the 
discovery and development of our product candidates. These expenses consist 
primarily of the following costs: compensation, benefits and other 
employee-related expenses, research and development related facilities, 
third-party contracts relating to nonclinical study and clinical trial 
activities. All research and development expenses are charged to operations as 
incurred.
Zagociguat is an orally administered CNS-penetrant sGC stimulator. NO-sGC-cGMP 
is a fundamental signaling network, including in the brain where it is 
critical to basic CNS functions. Deficient NO-sGC-cGMP signaling is believed 
to play an important role in the pathogenesis of many neurological disorders. 
As an sGC stimulator, zagociguat amplifies endogenous NO signaling by acting 
as a positive allosteric modulator to sensitize the sGC enzyme to NO, and 
increase the production of cGMP. By compensating for deficient NO-sGC-cGMP 
signaling, zagociguat may have broad therapeutic potential as a treatment for 
people with serious diseases.
In January 2020, we announced positive results from our Phase 1 first-in-human 
study that provided the first clinical data supporting the development of 
zagociguat. The results from this study indicate that zagociguat was well 
tolerated. Pharmacokinetic data, obtained from both blood and cerebral spinal 
fluid, support once-daily dosing with or without food and demonstrated 
zagociguat penetration of the blood-brain-barrier with concentrations in the 
CSF expected to be pharmacologically active.
In October 2020, we announced positive topline results from our zagociguat 
Phase 1 translational pharmacology study in healthy elderly participants. 
Treatment with zagociguat for 15 days in this 24-subject study confirmed and 
extended results seen in the earlier first-in-human Phase 1 study: once-daily 
oral treatment demonstrated blood-brain barrier penetration with expected CNS 
exposure and target engagement. Results also showed significant improvements 
in neurophysiological and objective performance measures as well as decreases 
in inflammatory biomarkers associated with aging and neurodegenerative 
diseases. Zagociguat was safe and generally well tolerated in the study. These 
results, together with nonclinical data, support continued development of 
zagociguat as a potential new medicine for serious CNS diseases.
In June 2022, we announced positive topline clinical data for zagociguat in 
our signal-seeking clinical study for the potential treatment of MELAS. In 
this open-label, single-arm study of the oral, once-daily sGC stimulator in 
eight adults aged 18 or older with MELAS, improvements were seen across a 
range of endpoints reflecting multiple domains of disease activity, including 
mitochondrial disease-associated biomarkers such as
                                                                                
                                       22                                       
-------------------------------------------------------------------------------

lactate and GDF-15, a broad panel of inflammatory biomarkers, cerebral blood 
flow, and functional connectivity between neural networks. These positive 
effects after 29 days of dosing were supported by correlations among several 
endpoints with each other and with zagociguat plasma concentrations. 
Zagociguat was well tolerated with no serious or severe adverse events and no 
events leading to discontinuation. Pharmacokinetics were consistent with the 
Phase 1 studies in healthy volunteers. The positive data from this study 
supports the potential of zagociguat to provide therapeutic benefit to people 
living with mitochondrial diseases, including MELAS.
In July 2022, we announced positive topline data from our signal-seeking 
clinical study of zagociguat for the potential treatment of CIAS. Data from 
the 14-day, double blind, randomized, placebo-controlled, multiple-ascending-dos
e study in 48 adults aged 18-50 with stable schizophrenia on a stable, single, 
atypical antipsychotic regimen demonstrated that once-daily zagociguat was 
safe and well tolerated, with no reports of serious adverse events, severe 
adverse events, or treatment discontinuation due to adverse events. We further 
announced that study data demonstrated a strong effect on cognitive 
performance after two weeks of 15mg once-daily dosing and that positive 
movement on inflammatory biomarkers was also observed. These signals on 
exploratory endpoints are consistent with the pro-cognitive and anti-inflammator
y effects of zagociguat observed in preclinical studies and prior clinical 
trials and support the further development of oral, once-daily zagociguat.
In October 2022, the WHO International Nonproprietary Names committee and the 
United States Adopted Name Council selected zagociguat as a nonproprietary 
name for CY6463.
In March 2023, we announced that given the significant capital and 
capabilities necessary to ensure that the MELAS Phase 2b study is executed 
efficiently and with the highest quality, and the currently unfavorable 
capital market conditions, we are actively evaluating the best combination of 
capital, capabilities, and transactions available to us to advance the 
development of zagociguat and our other clinical development candidates and to 
maximize shareholder value.
On March 31, 2023, we entered into a stock purchase agreement with the 
Companys Chief Executive Office ("CEO") pursuant to which the CEO will make an 
equity investment in the Company of $5 million in cash for common stock or 
nonvoting convertible preferred stock of the Company, the purchase price, 
consistent with Nasdaq rules, to be at or above the market price at the time 
of signing that agreement. The closing of the equity investment will take 
place six business days after the signing of the Asset Purchase Agreement, or 
May 11, 2023.
On May 11, 2023, the Company entered into an Asset Purchase Agreement (the 
"Asset Purchase Agreement") with an investor group which includes the CEO, JW 
Celtics Investment Corp. (Buyer Parent) and JW Cycler Inc. (Buyer). Pursuant 
to the terms of the Asset Purchase Agreement and subject to the approval by 
Company's shareholders, the Company will receive proceeds of $8 million as 
cash consideration, reimbursement for certain operating expenses related to 
zagociguat and CY3018 for the period between signing and closing of the 
transaction and 10% of all of Buyers Parent outstanding equity securities upon 
the successful closing of the transaction.
On May 11, 2023, we announced topline data from our signal- seeking clinical 
study of zagociguat for the potential treatment of Alzheimer's disease with 
vascular pathology ("ADv"), which study was supported in part by a $2 million 
grant from the Alzheimer's Associations Part the Cloud-Gates Partnership Grant 
Program (the "PTC Grant"). This exploratory, randomized, placebo-controlled, 
study of oral once-daily zagociguat was designed to evaluate safety, 
tolerability, and pharmacokinetics as well as explore the impact of zagociguat 
on biomarkers and cognitive performance over a twelve-week dosing period. The 
total number of enrolled participants was capped at 12 participants due to 
challenges associated with enrollment. Data from this study show that the 
safety and tolerability of profile once-daily zagociguat was consistent with 
prior studies. Given the small number of participants we are unable to draw 
any conclusions from the data generated in the study.
CY3018 is a CNS-targeted sGC stimulator in preclinical development that 
preferentially localizes to the brain and has a pharmacology profile that 
suggests its potential for the treatment of neuropsychiatric diseases and 
disorders.
Praliciguat is an orally administered, once-daily systemic sGC stimulator. On 
June 3, 2021, we entered into a license agreement with Akebia relating to the 
exclusive worldwide license to Akebia of our rights to the
                                                                                
                                       23                                       
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development, manufacture, medical affairs and commercialization of 
pharmaceutical products containing praliciguat and other related products and 
forms, thereof enumerated in such agreement. Cyclerion is eligible to receive 
up to $585 million in total potential future development, regulatory, and 
commercialization milestone payments. Cyclerion is also eligible to receive 
tiered, sales-based royalties ranging from single-digit to high-teen 
percentages.
Olinciguat is an orally administered, once-daily, vascular sGC stimulator that 
was evaluated in a Phase 2 study of participants with sickle cell disease. We 
released topline results from this study in October 2020. We intend to 
out-license olinciguat to an entity with strong cardiovascular and/or 
cardiopulmonary capabilities.
The following table summarizes our research and development expenses, employee 
and facility related costs allocated to research and development expense, and 
discovery and pre-clinical phase programs, for the three months ended March 
31, 2023 and 2022. The product pipeline expenses relate primarily to external 
costs associated with nonclinical studies and clinical trial costs, which are 
presented by development candidates.


                                                                 
                                           Three Months Ended    
                                                March 31,        
                                           2023           2022   
                                             (in thousands)      
Product pipeline external costs:                                 
Zagociguat                                  2,294          4,452 
CY3018                                         58            933 
Discovery research                             30            178 
Total product pipeline external costs       2,382          5,563 
Personnel and related internal costs        1,073          3,284 
Facilities and other                          318            896 
Total research and development expenses   $ 3,773        $ 9,743 


Securing regulatory approvals for new drugs is a lengthy and costly process. 
Any failure by us to obtain, or any delay in obtaining, regulatory approvals 
would materially adversely affect our product candidate development efforts 
and our business overall.
Given the inherent uncertainties of pharmaceutical product development, we 
cannot estimate with any degree of certainty how our programs will evolve, and 
therefore the amount of time or money that would be required to obtain 
regulatory approval to market them. As a result of these uncertainties 
surrounding the timing and outcome of any approvals, we are currently unable 
to estimate precisely when, if ever, our discovery and development candidates 
will be approved. We invest carefully in our pipeline, and the commitment of 
funding for each subsequent stage of our development programs is dependent 
upon the receipt of clear, supportive data.
The successful development of our product candidates is highly uncertain and 
subject to a number of risks including, but not limited to:
"
There is substantial doubt regarding our ability to continue as a going 
concern. We will need to raise additional funding, which may not be available 
on acceptable terms, or if at all. Failure to obtain necessary capital may 
force us to delay, limit or terminate our development efforts or other 
operations.
"
Cyclerion works closely with its clinical trial sites and investigators to 
deliver trials in a manner consistent with the safety of study participants 
and healthcare professionals.
"
The duration of clinical trials may vary substantially according to the type 
and complexity of the product candidate and may take longer than expected.
"
The United States FDA and comparable agencies outside the United States. 
impose substantial and varying requirements on the introduction of therapeutic 
pharmaceutical products, which typically require lengthy and detailed 
laboratory and clinical testing procedures, sampling activities and other 
costly and time-consuming procedures.
                                                                                
                                       24                                       
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"
Data obtained from nonclinical and clinical activities at any step in the 
testing process may be adverse and lead to discontinuation or redirection of 
development activity. Data obtained from these activities also are susceptible 
to varying interpretations, which could delay, limit or prevent regulatory 
approval.
"
The duration and cost of discovery, nonclinical studies and clinical trials 
may vary significantly over the life of a product candidate and are difficult 
to predict.
"
The costs, timing and outcome of regulatory review of a product candidate may 
not be favorable, and, even if approved, a product may face post-approval 
development and regulatory requirements.
"
The emergence of competing technologies and products and other adverse market 
developments may reduce or eliminate the potential value of our pipeline.
"
The continuing impact of COVID-19, which could continue to adversely affect 
our programs and operations, including our development activities, corporate 
development, and other activities.
As a result of the factors listed in the Risk Factors section in Part I, Item 
1A of our Annual Report on Form 10-K for the fiscal year ended December 31, 
2022, and elsewhere in this Quarterly Report on Form 10-Q, we are unable to 
determine the duration and costs to complete current or future nonclinical and 
clinical stages of our product candidates, including as licensed to third 
parties, or when, or to what extent, we may generate revenues from the 
commercialization and sale of our product candidates. Development timelines, 
probability of success and development costs vary widely. We anticipate that 
we will make determinations as to which additional programs to pursue and how 
much funding to direct to each program on an ongoing basis in response to the 
data from the studies of each product candidate, the competitive landscape and 
ongoing assessments of such product candidates commercial potential.
General and Administrative Expense.
General and administrative expenses consists primarily of compensation, 
benefits and other employee-related expenses for personnel in our 
administrative, finance, legal, information technology, business development, 
and human resource functions. Other costs include the legal costs of pursuing 
patent protection of our intellectual property, general and administrative 
related facility costs, insurance costs and professional fees for accounting 
and legal services. We record all general and administrative expenses as 
incurred.
Critical Accounting Policies and Estimates
Our discussion and analysis of our financial condition and results of 
operations is based upon our consolidated financial statements prepared in 
accordance with GAAP. The preparation of these financial statements requires 
us to make certain estimates and assumptions that may affect the reported 
amounts of assets and liabilities and disclosure of contingent assets and 
liabilities at the date of the consolidated financial statements, and the 
amounts of expenses during the reported periods. We base our estimates on our 
historical experience and on various other assumptions that are believed to be 
reasonable, the results of which form the basis for making judgments about the 
carrying values of assets and liabilities. Actual results may differ 
materially from our estimates under different assumptions or conditions. 
Changes in estimates are reflected in reported results in the period in which 
they become known.
We believe that our application of accounting policies requires significant 
judgments and estimates on the part of management and is the most critical to 
aid in fully understanding and evaluating our reported financial results. Our 
significant accounting policies are more fully described in Note 2,
Summary of Significant Accounting Policies
, of the consolidated financial statements elsewhere in this Quarterly Report 
on Form 10-Q.
All research and development expenses are expensed as incurred. We defer and 
capitalize nonrefundable advance payments we make for research and development 
activities until the related goods are received or the related services are 
performed. A discussion of our critical accounting policies and estimates may 
be found in our Annual Report on Form 10-K for the fiscal year ended December 
31, 2022 in Item 7,
Management's Discussion and Analysis of Financial Condition and Results of 
Operations
under the heading
Critical Accounting Policies and Estimates.
                                                                                
                                       25                                       
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Results of Operations
The revenue and expenses reflected in the consolidated financial statements 
may not be indicative of revenue and expenses that will be incurred by us in 
the future. The following discussion summarizes the key factors we believe are 
necessary for an understanding of our consolidated financial statements.
Revenues and Expenses


                                                                                          
                                             Three Months Ended            Change         
                                                  March 31,                               
                                             2023          2022          $          %     
                                                      (dollars in thousands)              
Revenues:                                                                                 
Revenue from development agreement                             225        (225 )   (100 )%
Revenue from grants                                            486        (486 )   (100 )%
Total revenues                                                 711        (711 )   (100 )%
Cost and expenses:                                                                        
Research and development                      3,773          9,743      (5,970 )    (61 )%
General and administrative                    3,269          3,952        (683 )    (17 )%
Total cost and expenses                       7,042         13,695      (6,653 )    (49 )%
Loss from operations                         (7,042 )      (12,984 )     5,942      (46 )%
Interest and other income (expenses), net        88              6          82     1367 % 
Net loss                                   $ (6,954 )    $ (12,978 )  $  6,024      (46 )%
                                                                                          


Revenues
. The decrease in revenue of approximately $0.7 million for the three months 
ended March 31, 2023, compared to the three months ended March 31, 2022, can 
be attributed to the revenue from the PTC Grant and the Akebia Supply 
Agreement recognized in 2022.
Research and development expense.
The decrease in research and development expense of approximately $6.0 million 
for the three months ended March 31, 2023 compared to the three months ended 
March 31, 2022 was driven by a decrease of approximately $2.2 million in 
external research costs related to the completion of the zagociguat clinical 
trials in CIAS and MELAS in 2022. a decrease of approximately $0.9 million for 
CY3018 costs, a decrease of approximately $0.1 million in discovery research, 
a decrease of approximately $1.6 million in other employee-related expenses 
primarily due to the workforce reduction in November 2022, a decrease of 
approximately $0.6 million in non-cash stock-based compensation, and a 
decrease of approximately $0.6 million in professional services.
General and administrative expense.
The decrease in general and administrative expenses of approximately $0.7 
million for the three months ended March 31, 2023 compared to the three months 
ended March 31, 2022 was primarily driven by a decrease in non-cash 
stock-based compensation.
Interest and other income (expenses), net.
Interest and other income (expenses), net increased by approximately $0.1 
million for the three months ended March 31, 2023, compared to the three 
months ended March 31, 2022 due to an increase in interest income driven by 
higher interest rates.
Liquidity and Capital Resources
On September 3, 2020, the Company entered into the Sales Agreement with 
Jefferies with respect to the ATM Offering under the Shelf. Under the ATM 
Offering, the Company may offer and sell, from time to time at its sole 
discretion, shares of its common stock, having an aggregate offering price of 
up to $50.0 million through Jefferies as its sales agent. The Company will pay 
Jefferies cash commissions of 3.0 percent of the gross proceeds of sales of 
common stock under the Sales Agreement. The Company has sold 3,353,059 shares 
of its common stock for net proceeds of $12.5 million under the ATM Offering 
since entering into the Sales Agreement, with no shares of common stock issued 
or sold under the ATM Offering during the three months ended March 31, 2023.
On June 7, 2021, we closed on a private placement of 5,735,988 shares of our 
common stock, pursuant to a Common Stock Purchase Agreement, for total gross 
proceeds of approximately $18 million. There were no material
                                                                                
                                       26                                       
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fees or commissions related to the transaction. The Company intends to use the 
proceeds to fund working capital and other general corporate purposes.
Our ability to continue to fund our operations and meet capital needs will 
depend on our ability to generate cash from operations and access to capital 
markets and other sources of capital, as further described below. We 
anticipate that our principal uses of cash in the future will be primarily to 
fund our operations, working capital needs, capital expenditures and other 
general corporate purposes.
On March 31, 2023, we had approximately $7.2 million of unrestricted cash and 
cash equivalents. Our cash equivalents include amounts held in U.S. government 
money market funds. We invest cash in excess of immediate requirements in 
accordance with our investment policy, which requires all investments held by 
us to be at least AAA rated or equivalent, with a remaining final maturity 
when purchased of less than twelve months, so as to primarily achieve 
liquidity and capital preservation.
Going Concern
The Company evaluated whether there are conditions and events, considered in 
the aggregate, that raise substantial doubt about its ability to continue as a 
going concern within one year after the date that these consolidated financial 
statements are issued. This evaluation initially does not take into 
consideration the potential mitigating effect of managements plans that have 
not been fully implemented as of the date the financial statements are issued. 
When substantial doubt exists under this methodology, management evaluates 
whether the mitigating effect of its plans sufficiently alleviates substantial 
doubt about the Companys ability to continue as a going concern. The 
mitigating effect of managements plans, however, is only considered if both 
(1) it is probable that the plans will be effectively implemented within one 
year after the date that the financial statements are issued, and (2) it is 
probable that the plans, when implemented, will mitigate the relevant 
conditions or events that raise substantial doubt about the entitys ability to 
continue as a going concern within one year after the date that these 
consolidated financial statements are issued. In performing its analysis, 
management excluded certain elements of its operating plan that cannot be 
considered probable. Under ASC 205-40, the future receipt of potential funding 
from future partnerships, equity or debt issuances, and the potential 
milestones from the Akebia agreement cannot be considered probable at this 
time because these plans are not entirely within the Companys control and/or 
have not been approved by the Board of Directors as of the date of these 
consolidated financial statements.
The Company has incurred recurring losses since its inception, including a net 
loss of $7.0 million for the three months ended March 31, 2023. In addition, 
as of March 31, 2023, the Company had an accumulated deficit of $266.1 
million. The Company expects to continue to generate operating losses for the 
foreseeable future. The Company expects that its cash, cash equivalents and 
marketable securities as of March 31, 2023 will not be sufficient to fund 
operations for at least the next twelve months from the date of issuance of 
these consolidated financial statements and the Company will need to obtain 
additional funding. Accordingly, the Company has concluded that substantial 
doubt exists about the Companys ability to continue as a going concern for a 
period of at least 12 months from the date of issuance of these consolidated 
financial statements.
The accompanying financial statements have been prepared on a going concern 
basis, which contemplates the realization of assets and satisfaction of 
liabilities in the ordinary course of business. The financial statements do 
not include any adjustments relating to the recoverability and classification 
of recorded asset amounts or the amounts and classification of liabilities 
that might result from the outcome of the uncertainties described above.

Continued Nasdaq Listing
On June 1, 2022, the Company received a notice from the Nasdaq Stock Market 
("Nasdaq") notifying the Company that, for the last 30 consecutive business 
days, the closing bid price for the Company's common stock listed on Nasdaq 
has been below the minimum $1.00 per share required for continued listing on 
the Nasdaq Global Select Market pursuant to Nasdaq Listing Rule 5450(a)(1) 
(the "Bid Price Requirement").
In accordance with Nasdaq Listing Rule 5810(c)(3)(A), the Company was provided 
a period of 180 calendar days, or until November 28, 2022, to regain 
compliance with the Bid Price Requirement. The Company did not regain 
compliance with the Bid Price Requirement by the initial compliance date. On 
November 29, 2022,
                                                                                
                                       27                                       
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Nasdaq notified the Company that it is eligible for an additional 180 calendar 
day period, or until May 29, 2023 (the "Extended Compliance Date"), to regain 
compliance with the Bid Price Requirement. Nasdaqs determination was based on 
the Company meeting the continued listing requirement for market value of 
publicly held shares and all other applicable requirements for initial listing 
on the Nasdaq Capital Market with the exception of the Bid Price Requirement, 
and the Companys written notice of its intention to cure the deficiency during 
the second compliance period by effecting a reverse stock split, if necessary. 
Effective November 25, 2022, the Company transferred its listing of the 
Companys common stock from the Nasdaq Global Market to the Nasdaq Capital 
Market, a continuous trading market that operates in substantially the same 
manner as the Nasdaq Global Market. The Companys common stock continues to 
trade under the symbol CYCN.
To date, the Company has not regained compliance with the Bid Price 
Requirement. If at any time before May 29, 2023, the bid price of the 
Company's common stock closes at a $1.00 per share or more for a minimum of 10 
consecutive business days, Nasdaq will provide written notification to the 
Company that it has regained compliance with the Bid Price Requirement. If the 
Company does not regain compliance with the Bid Price Requirement by the end 
of the second compliance period, the Company's stock will be subject to 
delisting.
On April 3, 2023, the Company filed with the SEC a definitive proxy statement 
for its annual meeting of stockholders to be held on May 15, 2023, at which 
meeting the Company is seeking stockholder approval of a reverse stock split 
with the primary intent of increasing the price of its common stock to meet 
the price criteria for continued listing on Nasdaq. The Company intends to 
monitor the closing bid price of its common stock and may, if appropriate, 
consider available options to regain compliance with the Bid Price 
Requirement, including initiating a reverse stock split. However, there can be 
no assurance on how stockholders will vote on such proposal or that the 
Company will be able to regain compliance with the Bid Price Requirement or 
will otherwise be in compliance with other Nasdaq Listing Rules.
Cash Flows
The following is a summary of cash flows for the three months ended March 31, 
2023 and 2022:


                                                                                   
                                         Three Months Ended           Change       
                                              March 31,                            
                                         2023          2022          $       %     
                                                (dollars in thousands)             
Net cash used in operating activities  $ (6,214 )    $ (12,835 )  $ 6,621    (52 )%


Cash Flows from Operating Activities
Net cash used in operating activities was $6.2 million for the three months 
ended March 31, 2023 compared to $12.8 million for the three months ended 
March 31, 2022. The decrease in net cash used in operations of $6.6 million 
primarily relates to a decrease in our net loss of $6.0 million, a decrease in 
working capital accounts of $2.0 million, partially offset by a decrease of 
stock-based compensation of $1.4 million.
Funding Requirements
We expect our expenses to fluctuate as we engage in preclinical activities and 
clinical trials of our product candidates, continue to maintain out-license 
opportunities and seek to broaden our portfolio through in-licensing of 
complementary CNS assets. Based on our cash and cash equivalents position as 
of March 31, 2023 and our planned operating expenses and capital expenditure 
requirements there is substantial doubt regarding our ability to continue as a 
going concern for a period of one year after the date of this Quarterly Report 
on Form 10-Q. We will need to raise additional capital in upcoming periods 
which may not be available on acceptable terms, if at all. Failure to obtain 
necessary capital when needed may delay current development of our product 
candidates, halt new development phases, or other operations.
Because of the many risks and uncertainties associated with research, 
development and commercialization of product candidates, we are unable to 
estimate the exact amount of our working capital requirements. Our
                                                                                
                                       28                                       
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expenses will fluctuate, and our future funding requirements will depend on, 
and could increase or decrease significantly as a result of many factors 
including the:
"
scope, progress, results and costs of researching and developing our product 
candidates, and conducting preclinical studies and clinical trials;
"
costs, timing and outcome of regulatory review of our product candidates;
"
costs of future activities, including medical affairs, manufacturing and 
distribution, for any of our product candidates for which we receive marketing 
approval;
"
cost and timing of necessary actions to support our strategic objectives;
"
costs of preparing, filing and prosecuting patent applications, maintaining 
and enforcing our intellectual property rights and defending intellectual 
property-related claims; and
"
timing, receipt and amount of sales of, or milestone payments related to or 
royalties on, our current or future product candidates, if any.
A change in any of these or other variables with respect to the development of 
any of our product candidates could significantly change the costs and timing 
of the development of that product candidate.
Until such time, if ever, as we can generate substantial product revenue, we 
expect to finance our cash needs through a combination of public or private 
equity offerings, debt financings, collaborations, strategic alliances or 
licensing arrangements with third parties. To the extent that we raise 
additional capital through the sale of equity or convertible debt securities, 
outstanding equity ownership may be materially diluted, and the terms of 
securities sold in such transactions could include liquidation or other 
preferences that adversely affect the rights of holders of common stock. Debt 
financing and preferred equity financing, if available, may involve agreements 
that include restrictive covenants that limit our ability to take specified 
actions, such as incurring additional debt, making capital expenditures or 
declaring dividends. In addition, debt financing would result in increased 
fixed payment obligations.
If we raise funds through collaborations, strategic alliances or licensing 
arrangements with third parties, we may have to relinquish valuable rights to 
our technologies, future revenue streams, research programs or product 
candidates or grant licenses on terms that may not be favorable to us.
If we are unable to raise additional funds when needed, we may be required to 
delay, reduce or eliminate our product development or future commercialization 
efforts, or grant rights to develop and market product candidates that we 
would otherwise prefer to develop and market ourselves.
Contractual Commitments and Obligations
Tax-related Obligations
We exclude assets, liabilities or obligations pertaining to uncertain tax 
positions from our summary of contractual commitments and obligations as we 
cannot make a reliable estimate of the period of cash settlement with the 
respective taxing authorities. As of March 31, 2023, we had no uncertain tax 
positions.
Other Funding Commitments
As of March 31, 2023, we had, and continue to have, several ongoing studies in 
various clinical trial stages. Our most significant clinical trial spending is 
with clinical research organizations, or CROs. The contracts with CROs 
generally are cancellable, with notice, at our option and do not have any 
significant cancellation penalties.
Off-Balance Sheet Arrangements
We do not have any relationships with unconsolidated entities or financial 
partnerships, such as entities often referred to as structured finance or 
special purpose entities, that would have been established for the purpose of 
facilitating off-balance sheet arrangements (as that term is defined in Item 
303(a)(4)(ii) of Regulation S-K) or other contractually narrow or limited 
purposes. As such, we are not exposed to any financing, liquidity, market or 
credit risk that could arise if we had engaged in those types of relationships. 
We enter into guarantees in the ordinary course of business related to the 
guarantee of our own performance.
                                                                                
                                       29                                       
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New Accounting Pronouncements
For a discussion of new accounting pronouncements see Note 2,
Summary of Significant Accounting Policies
, of the consolidated financial statements appearing elsewhere in this 
Quarterly Report on Form 10-Q.
Item 3. Quantitative and Qualitati
ve Disclosures About Market Risk.
We are a smaller reporting company as defined by Rule 12b-2 of the Securities 
Exchange Act of 1934, as amended (the Exchange Act) and are not required to 
provide the information required under this item.
Item 4. Controls
and Procedures.
Evaluation of Disclosure Controls and Procedures
The term disclosure controls and procedures, as defined in Rules 13a-15(e) and 
15d-15(e) under the Exchange Act refers to controls and procedures that are 
designed to ensure that information required to be disclosed by a company in 
the reports that it files or submits under the Exchange Act is recorded, 
processed, summarized and reported within the time periods specified in the 
SECs rules and forms. Disclosure controls and procedures include, without 
limitation, controls and procedures designed to ensure that information 
required to be disclosed by a company in the reports that it files or submits 
under the Exchange Act is accumulated and communicated to the companys 
management, including its principal executive and principal financial 
officers, or persons performing similar functions, as appropriate to allow 
timely decisions regarding required disclosure. Because there are inherent 
limitations in all control systems, a control system, no matter how well 
conceived and operated, can provide only reasonable, as opposed to absolute, 
assurance that the objectives of the control system are met. These inherent 
limitations include the realities that judgments in decision-making can be 
faulty, and that breakdowns can occur because of simple error or mistake. 
Additionally, controls can be circumvented by the individual acts of some 
persons, by collusion of two or more people, or by management override of the 
control. Further, the design of a control system must reflect the fact that 
there are resource constraints, and the benefits of controls must be 
considered relative to their costs. Our management, with the participation of 
our chief executive officer and chief financial officer, evaluated the 
effectiveness of our disclosure controls and procedures as of the end of the 
period covered by this report. Based on that evaluation, our chief executive 
officer and chief financial officer concluded that our disclosure controls and 
procedures were effective, at the reasonable assurance level, as of the end of 
the period covered by this report. However, our management does not expect 
that our disclosure controls and procedures or our internal control over 
financial reporting will prevent all errors and all fraud.
Changes in Internal Control over Financial Reporting
There were no changes in our internal control over financial reporting (as 
defined in Rules 13a-15(f) and 15d-15(f) under the Exchange Act) that occurred 
during the period covered by this Quarterly Report on Form 10-Q which have 
materially affected, or are reasonably likely to materially affect, our 
internal control over financial reporting.
                                                                                
                                       30                                       
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                                      PART                                      
                                       II                                       
Item 1.
Legal
Proceedings
We are not a party to any material legal proceedings at this time. From time 
to time we may be subject to various legal proceedings and claims, which may 
have a material adverse effect on our financial position or results of 
operations.
Item 1A.
Ri
sk Factors
You should carefully review and consider the information regarding certain 
factors which could materially affect our business, financial condition or 
future results set forth under the heading Risk Factors in Part I, Item 1A of 
our Annual Report on Form 10-K for the fiscal year ended December 31, 2022. 
There have been no material changes from the risk factors previously disclosed 
therein.
Ite
m 5.
Other Information
Not applicable.
Item 6.
Exhibits
See the Exhibit Index on the following page of this Quarterly Report on Form 
10-Q.
                                                                                
                                       31                                       
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                                 EXHIBIT INDEX                                  


                                                                                                  
Exhibit  Description                                                                              
No.                                                                                               
                                                                                                  
10.1     Stock Purchase Agreement, incorporated by reference to Exhibit 1 to                      
         Amendment No. 3 to Schedule 13D filed by Peter M. Hecht on April 3, 2023                 
31.1     Certificate of Chief Executive Officer (Principal Executive                              
         Officer) pursuant to Section 302 of the Sarbanes-Oxley Act of 2002                       
31.2     Certificate of Chief Financial Officer (Principal Financial                              
         Officer) pursuant to Section 302 of the Sarbanes-Oxley Act of 2002                       
32.1     Certificate of Chief Executive Officer (Principal Executive Officer) pursuant to 18      
         U.S.C. Section 1350, as adopted pursuant to Section 906 of the Sarbanes-Oxley Act of 2002
32.2     Certificate of Chief Financial Officer (Principal Financial Officer) pursuant to 18      
         U.S.C. Section 1350, as adopted pursuant to Section 906 of the Sarbanes-Oxley Act of 2002
101.INS  Inline XBRL Instance Document.                                                           
101.SCH  Inline XBRL Taxonomy                                                                     
         Extension Schema Document.                                                               
101.CAL  Inline XBRL Taxonomy Extension                                                           
         Calculation Linkbase Document.                                                           
101.DEF  Inline XBRL Taxonomy Extension                                                           
         Definition Linkbase Document.                                                            
101.LAB  Inline XBRL Taxonomy Extension                                                           
         Label Linkbase Document.                                                                 
101.PRE  Inline XBRL Taxonomy Extension                                                           
         Presentation Linkbase Document.                                                          
104      Cover Page Interactive Data File.                                                        



                                                                                
                                       32                                       
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                                     SIGNA                                      
                                     TURES                                      
Pursuant to the requirements of the Securities Exchange Act of 1934, the 
registrant has duly caused this report to be signed on its behalf by the 
undersigned, thereunto duly authorized.


                                                                            
                                                CYCLERION THERAPEUTICS, INC.
                                                                            
    By:                                                   /s/ Peter M. Hecht
  Name:                                                       Peter M. Hecht
 Title:                Chief Executive Officer (Principal Executive Officer)
                                                                            
    By:                                                     /s/ Anjeza Gjino
  Name:                                                         Anjeza Gjino
 Title: Chief Financial Officer (Principal Financial and Accounting Officer)


Date: May 11, 2023
                                                                                
                                       33                                       
-------------------------------------------------------------------------------

                                                                    Exhibit 31.1
                  CERTIFICATION OF PRINCIPAL EXECUTIVE OFFICER                  
           PURSUANT TO SECTION 302 OF THE SARBANES-OXLEY ACT OF 2002            
I, Peter M. Hecht, certify that:
1. I have reviewed this quarterly report on Form 10-Q of Cyclerion 
Therapeutics, Inc.;
2. Based on my knowledge, this report does not contain any untrue statement of 
a material fact or omit to state a material fact necessary to make the 
statements made, in light of the circumstances under which such statements 
were made, not misleading with respect to the period covered by this report;

3. Based on my knowledge, the financial statements, and other financial 
information included in this report, fairly present in all material respects 
the financial condition, results of operations and cash flows of the 
registrant as of, and for, the periods presented in this report;
4. The registrants other certifying officer and I are responsible for 
establishing and maintaining disclosure controls and procedures (as defined in 
Exchange Act Rules 13a-15(e) and 15d-15(e)) and internal control over 
financial reporting (as defined in Exchange Act Rules 13a-15(f) and 15d-15(f)) 
for the registrant and have:
(a) Designed such disclosure controls and procedures, or caused such 
disclosure controls and procedures to be designed under our supervision, to 
ensure that material information relating to the registrant, including its 
consolidated subsidiaries, is made known to us by others within those 
entities, particularly during the period in which this report is being 
prepared;
(b) Designed such internal control over financial reporting, or caused such 
internal control over financial reporting to be designed under our 
supervision, to provide reasonable assurance regarding the reliability of 
financial reporting and the preparation of financial statements for external 
purposes in accordance with generally accepted accounting principles;
(c) Evaluated the effectiveness of the registrants disclosure controls and 
procedures and presented in this report our conclusions about the 
effectiveness of the disclosure controls and procedures, as of the end of the 
period covered by this report based on such evaluation; and
(d) Disclosed in this report any change in the registrants internal control 
over financial reporting that occurred during the registrants most recent 
fiscal quarter (the registrants fourth fiscal quarter in the case of an annual 
report) that has materially affected, or is reasonably likely to materially 
affect, the registrants internal control over financial reporting; and
5. The registrants other certifying officer and I have disclosed, based on our 
most recent evaluation of internal control over financial reporting, to the 
registrants auditors and the audit committee of the registrants board of 
directors (or persons performing the equivalent functions):
(a) All significant deficiencies and material weaknesses in the design or 
operation of internal control over financial reporting which are reasonably 
likely to adversely affect the registrants ability to record, process, 
summarize and report financial information; and
(b) Any fraud, whether or not material, that involves management or other 
employees who have a significant role in the registrants internal control over 
financial reporting.


                                                                               
Date: May 11, 2023    By:                                    /s/ Peter M. Hecht
                    Name:                                        Peter M. Hecht
                   Title: Chief Executive Officer (Principal Executive Officer)



-------------------------------------------------------------------------------

                                                                    Exhibit 31.2
                  CERTIFICATION OF PRINCIPAL FINANCIAL OFFICER                  
           PURSUANT TO SECTION 302 OF THE SARBANES-OXLEY ACT OF 2002            
I, Anjeza Gjino, certify that:
1. I have reviewed this quarterly report on Form 10-Q of Cyclerion 
Therapeutics, Inc.;
2. Based on my knowledge, this report does not contain any untrue statement of 
a material fact or omit to state a material fact necessary to make the 
statements made, in light of the circumstances under which such statements 
were made, not misleading with respect to the period covered by this report;

3. Based on my knowledge, the financial statements, and other financial 
information included in this report, fairly present in all material respects 
the financial condition, results of operations and cash flows of the 
registrant as of, and for, the periods presented in this report;
4. The registrants other certifying officer and I are responsible for 
establishing and maintaining disclosure controls and procedures (as defined in 
Exchange Act Rules 13a-15(e) and 15d-15(e)) and internal control over 
financial reporting (as defined in Exchange Act Rules 13a-15(f) and 15d-15(f)) 
for the registrant and have:
(a) Designed such disclosure controls and procedures, or caused such 
disclosure controls and procedures to be designed under our supervision, to 
ensure that material information relating to the registrant, including its 
consolidated subsidiaries, is made known to us by others within those 
entities, particularly during the period in which this report is being 
prepared;
(b) Designed such internal control over financial reporting, or caused such 
internal control over financial reporting to be designed under our 
supervision, to provide reasonable assurance regarding the reliability of 
financial reporting and the preparation of financial statements for external 
purposes in accordance with generally accepted accounting principles;
(c) Evaluated the effectiveness of the registrants disclosure controls and 
procedures and presented in this report our conclusions about the 
effectiveness of the disclosure controls and procedures, as of the end of the 
period covered by this report based on such evaluation; and
(d) Disclosed in this report any change in the registrants internal control 
over financial reporting that occurred during the registrants most recent 
fiscal quarter (the registrants fourth fiscal quarter in the case of an annual 
report) that has materially affected, or is reasonably likely to materially 
affect, the registrants internal control over financial reporting; and
5. The registrants other certifying officer and I have disclosed, based on our 
most recent evaluation of internal control over financial reporting, to the 
registrants auditors and the audit committee of the registrants board of 
directors (or persons performing the equivalent functions):
(a) All significant deficiencies and material weaknesses in the design or 
operation of internal control over financial reporting which are reasonably 
likely to adversely affect the registrants ability to record, process, 
summarize and report financial information; and
(b) Any fraud, whether or not material, that involves management or other 
employees who have a significant role in the registrants internal control over 
financial reporting.


                                                                                              
Date: May 11, 2023    By:                                                     /s/ Anjeza Gjino
                    Name:                                                         Anjeza Gjino
                   Title: Chief Financial Officer (Principal Financial and Accounting Officer)



-------------------------------------------------------------------------------

                                                                    Exhibit 32.1
                    CERTIFICATION OF CHIEF EXECUTIVE OFFICER                    
                      PURSUANT TO 18 U.S.C. SECTION 1350,                       
      AS ADOPTED PURSUANT TO SECTION 906 OF THE SARBANES-OXLEY ACT OF 2002      
I, Peter M. Hecht, certify pursuant to 18 U.S.C. Section 1350, as adopted 
pursuant to Section 906 of the Sarbanes-Oxley Act of 2002, that, to my 
knowledge, the Quarterly Report on Form 10-Q of Cyclerion Therapeutics, Inc. 
for the period ended March 31, 2023 fully complies with the requirements of 
Section 13(a) or 15(d) of the Securities Exchange Act of 1934 and the 
information contained in such Form 10-Q fairly presents, in all material 
respects, the financial condition and results of operations of Cyclerion 
Therapeutics, Inc.


                                                                               
Date: May 11, 2023    By:                                    /s/ Peter M. Hecht
                    Name:                                        Peter M. Hecht
                   Title: Chief Executive Officer (Principal Executive Officer)



-------------------------------------------------------------------------------

                                                                    Exhibit 32.2
                    CERTIFICATION OF CHIEF FINANCIAL OFFICER                    
                      PURSUANT TO 18 U.S.C. SECTION 1350,                       
      AS ADOPTED PURSUANT TO SECTION 906 OF THE SARBANES-OXLEY ACT OF 2002      
I, Anjeza Gjino, certify pursuant to 18 U.S.C. Section 1350, as adopted 
pursuant to Section 906 of the Sarbanes-Oxley Act of 2002, that, to my 
knowledge, the Quarterly Report on Form 10-Q of Cyclerion Therapeutics, Inc. 
for the period ended March 31, 2023 fully complies with the requirements of 
Section 13(a) or 15(d) of the Securities Exchange Act of 1934 and the 
information contained in such Form 10-Q fairly presents, in all material 
respects, the financial condition and results of operations of Cyclerion 
Therapeutics, Inc.


                                                                                              
Date: May 11, 2023    By:                                                     /s/ Anjeza Gjino
                    Name:                                                         Anjeza Gjino
                   Title: Chief Financial Officer (Principal Financial and Accounting Officer)



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